Characterization of β-glucosidase from corn stover and its application in simultaneous saccharification and fermentation

被引：55

作者：

Han, Yejun ^{[1
]}

Chen, Hongzhang ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 14期

关键词：

beta-glucosidase; characterization; optimum temperatures; corn stover; simultaneous saccharification and fermentation (SSF);

D O I：

10.1016/j.biortech.2007.12.050

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Purification and characterization of beta-glucosidase from corn stover was performed and the enzyme was tried in SSF to evaluate the suitability of plant glycosyl hydrolases in lignocellulose conversion. A beta-glucosidase with M-w of 62.4 kDa was purified to homogeneity from post-harvest corn stover. The following physicochemical and kinetic parameters of the beta-glucosidase were studied respectively: optimum temperature, thermal stability, optimum pH, pH stability, K-m, V-max, V-i, cellobiose inhibition, tryptic peptide mass spectrometry and effect of metal ions and other reagents on the activity. The beta-glucosidase activity on salicin was optimal at pH 4.8 and 37 degrees C. The unique property of optimum temperature makes the beta-glucosidase potentially useful in SSF. In SSF of steam explosion pretreated corn stover, the supplementation of the purified beta-glucosidase was more effective than Aspergillus niger beta-glucosidase. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：6081 / 6087

页数：7

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