Translational activation by the noncoding RNA DsrA involves alternative RNase III processing in the rpoS 5′-leader

被引：49

作者：

Resch, Armin ^{[1
]}

Afonyushkin, Taras ^{[1
]}

Lombo, Tania B. ^{[1
]}

McDowall, Kenneth J. ^{[2
]}

Blaesi, Udo ^{[1
]}

Kaberdin, Vladimir R. ^{[1
]}

机构：

[1] Univ Dept Vienna Bioctr, Dept Microbiol & Immunobiol, Max F Perutz Labs, A-1030 Vienna, Austria

[2] Univ Leeds, Fac Biol Sci, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

RNA | 2008年 / 14卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 奥地利科学基金会;

关键词：

DsrA; mRNA stability; noncoding RNA; rpoS;

D O I：

10.1261/rna.603108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intricate regulation of the Escherichia coli rpoS gene, which encodes the stationary phase sigma-factor sigma(S), includes translational activation by the noncoding RNA DsrA. We observed that the stability of rpoS mRNA, and concomitantly the concentration of sS, were significantly higher in an RNase III-deficient mutant. As no decay intermediates corresponding to the in vitro mapped RNase III cleavage site in the rpoS leader could be detected in vivo, the initial RNase III cleavage appears to be decisive for the observed rapid inactivation of rpoS mRNA. In contrast, we show that base-pairing of DsrA with the rpoS leader creates an alternative RNase III cleavage site within the rpoS/DsrA duplex. This study provides new insights into regulation by small regulatory RNAs in that the molecular function of DsrA not only facilitates ribosome loading on rpoS mRNA, but additionally involves an alternative processing of the target.

引用

页码：454 / 459

页数：6

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