PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells

被引：30

作者：

Zhou, CJ

Yada, T

Kohno, D

Kikuyama, S

Suzuki, R

Mizushima, H

Shioda, S

机构：

[1] Showa Univ, Sch Med, Dept Anat, Shinagawa Ku, Tokyo 1428555, Japan

[2] Waseda Univ, Sch Educ, Dept Biol, Shinjuku Ku, Tokyo 1698050, Japan

[3] Jichi Med Sch, Dept Physiol, Minami Kawachi, Tochigi 3290498, Japan

来源：

PEPTIDES | 2001年 / 22卷 / 07期

基金：

日本科学技术振兴机构;

关键词：

PACAP; G-protein-coupled receptor; alternative splicing variants; cAMP/protein kinase A cascades; phospholipase C/protein kinase C cascade cytosolic Ca2+ concentration; development; neural fold; primitive streak stage; rat;

D O I：

10.1016/S0196-9781(01)00437-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several studies have reported that the PAC, receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+](i)) was increased by PACAP, but not VIP, in a concentration range from 10(-12) to 10(-8) M via the PAC(1)-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC(1)-R is linked to [Ca2+](i) signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：1111 / 1117

页数：7

共 31 条

[1] PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) AND ITS RECEPTORS - NEUROENDOCRINE AND ENDOCRINE INTERACTION [J].