LZF1, a HY5-regulated transcriptional factor, functions in Arabidopsis de-etiolation

被引:161
作者
Chang, Chiung-Swey Joanne [1 ,2 ,4 ,5 ]
Li, Yi-Hang [1 ]
Chen, Li-Teh [1 ]
Chen, Wan-Chieh [1 ]
Hsieh, Wen-Ping [1 ]
Shin, Jieun [3 ]
Jane, Wann-Neng [1 ]
Chou, Shu-Jen [1 ]
Choi, Giltsu [3 ]
Hu, Jer-Ming [6 ]
Somerville, Shauna [7 ]
Wu, Shu-Hsing [1 ,2 ,4 ,5 ]
机构
[1] Acad Sinica, Inst Plant & Microbial Biol, Taipei 11529, Taiwan
[2] Natl Chung Hsing Univ, Taiwan Int Grad Program, Mol & Biol Agr Sci Program, Taipei 11529, Taiwan
[3] Korea Adv Inst Sci & Technol, Dept Sci Biol, Taejon 305701, South Korea
[4] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 402, Taiwan
[5] Natl Chung Hsing Univ, Dept Life Sci, Taichung 402, Taiwan
[6] Natl Taiwan Univ, Inst Ecol & Evolut Biol, Taipei 106, Taiwan
[7] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA
关键词
light; HY5; LZF1; chlorophyll; anthocyanin; arabidopsis;
D O I
10.1111/j.1365-313X.2008.03401.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We surveyed differential gene expression patterns during early photomorphogenesis in both wild-type and mutant Arabidopsis defective in HY5, an influential positive regulator of the responses of gene expression to a light stimulus, to identify light-responsive genes whose expression was HY5 dependent. These gene-expression data identified light-regulated zinc finger protein 1 (LZF1), a gene encoding a previously uncharacterized C2C2-CO B-box transcriptional regulator. HY5 has positive trans-activating activity toward LZF1 and binding affinity to LZF1 promoter in vivo. HY5 is needed but not sufficient for the induction of LZF1 expression. Anthocyanin content is significantly diminished in lzf1 under far red, which is the most efficient light for the induction of LZF1. The expression of PAP1/MYB75 is elevated in plants overexpressing LZF1, which leads to the hyperaccumulation of anthocyanin in transgenic Arabidopsis. The transition from etioplast to chloroplast and the accumulation of chlorophyll were notably compromised in the lzf1 mutant. We provide molecular evidence that LZF1 influences chloroplast biogenesis and function via regulating genes encoding chloroplast proteins. In the absence of HY5, mutation of LZF1 leads to further reduced light sensitivity for light-regulated inhibition of hypocotyl elongation and anthocyanin and chlorophyll accumulation. Our data indicate that LZF1 is a positive regulator functioning in Arabidopsis de-etiolation.
引用
收藏
页码:205 / 219
页数:15
相关论文
共 80 条
[1]   Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].
Alonso, JM ;
Stepanova, AN ;
Leisse, TJ ;
Kim, CJ ;
Chen, HM ;
Shinn, P ;
Stevenson, DK ;
Zimmerman, J ;
Barajas, P ;
Cheuk, R ;
Gadrinab, C ;
Heller, C ;
Jeske, A ;
Koesema, E ;
Meyers, CC ;
Parker, H ;
Prednis, L ;
Ansari, Y ;
Choy, N ;
Deen, H ;
Geralt, M ;
Hazari, N ;
Hom, E ;
Karnes, M ;
Mulholland, C ;
Ndubaku, R ;
Schmidt, I ;
Guzman, P ;
Aguilar-Henonin, L ;
Schmid, M ;
Weigel, D ;
Carter, DE ;
Marchand, T ;
Risseeuw, E ;
Brogden, D ;
Zeko, A ;
Crosby, WL ;
Berry, CC ;
Ecker, JR .
SCIENCE, 2003, 301 (5633) :653-657
[2]   Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development [J].
Ang, LH ;
Chattopadhyay, S ;
Wei, N ;
Oyama, T ;
Okada, K ;
Batschauer, A ;
Deng, XW .
MOLECULAR CELL, 1998, 1 (02) :213-222
[3]  
[Anonymous], 1992, Statistical Science, DOI DOI 10.1214/SS/1177011454
[4]   LAF1, a MYB transcription activator for phytochrome A signaling [J].
Ballesteros, ML ;
Bolle, C ;
Lois, LM ;
Moore, JM ;
Vielle-Calzada, JP ;
Grossniklaus, U ;
Chua, NH .
GENES & DEVELOPMENT, 2001, 15 (19) :2613-2625
[5]   Far-red light blocks greening of arabidopsis seedlings via a phytochrome A-mediated change in plastid development [J].
Barnes, SA ;
Nishizawa, NK ;
Quaggio, RB ;
Whitelam, GC ;
Chua, NH .
PLANT CELL, 1996, 8 (04) :601-615
[6]   Requirement of aminoacyl-tRNA synthetases for gametogenesis and embryo development in Arabidopsis [J].
Berg, M ;
Rogers, R ;
Muralla, R ;
Meinke, D .
PLANT JOURNAL, 2005, 44 (05) :866-878
[7]   CUL4 associates with DDB1 and DET1 and its downregulation affects diverse aspects of development in Arabidopsis thaliana [J].
Bernhardt, Anne ;
Lechner, Esther ;
Hano, Perdita ;
Schade, Verena ;
Dieterle, Monika ;
Anders, Marco ;
Dubin, Manu Jody ;
Benvenuto, Giovanna ;
Bowler, Chris ;
Genschik, Pascal ;
Hellmann, Hanjo .
PLANT JOURNAL, 2006, 47 (04) :591-603
[8]   Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis [J].
Borevitz, JO ;
Xia, YJ ;
Blount, J ;
Dixon, RA ;
Lamb, C .
PLANT CELL, 2000, 12 (12) :2383-2393
[9]  
Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468
[10]   Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression [J].
Chattopadhyay, S ;
Ang, LH ;
Puente, P ;
Deng, XW ;
Wei, N .
PLANT CELL, 1998, 10 (05) :673-683