Notochordal cells interact with nucleus pulposus cells: Regulation of proteoglycan synthesis

被引:258
作者
Aguiar, DJ
Johnson, SL
Oegema, TR
机构
[1] Univ Minnesota, Dept Orthopaed Surg, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Biochem, Minneapolis, MN 55455 USA
关键词
nucleus pulposus; notochord cells; proteoglycan; intervertebral disc;
D O I
10.1006/excr.1998.4287
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The disappearance of notochordal cells is correlated with early degenerative changes in the intervertebral disc, With increased disc degeneration there is a marked decrease in proteoglycan synthesis, resulting in loss of mechanical function. One possible mechanism for the decrease in proteoglycan synthesis is the loss of notochordal cells from the tissue. In this study, nucleus pulposus cells cocultured with notochordal cells exhibit an increase in proteoglycan synthesis. Interestingly, purified notochordal cells synthesize little proteoglycan as observed by [S-35]sulfate incorporation into proteoglycans. The observed increase in proteoglycan synthesis does not appear to be dependent on cell-cell contact; rather it is the result of soluble factor(s) produced by notochordal cells. Finally, no difference in chondroitin sulfate chain size in notochordal-stimulated nucleus pulposus cells was observed which is consistent with an up-regulation in aggrecan core protein synthesis. These results are consistent with canine breeds where notochordal cells persist into adult age and disc degeneration is not observed. This suggests notochordal cells play a vital role in maintaining disc integrity. (C) 1999 Academic Press.
引用
收藏
页码:129 / 137
页数:9
相关论文
共 32 条
  • [1] PROTEOGLYCAN SYNTHESIS IN THE HUMAN INTERVERTEBRAL-DISK - VARIATION WITH AGE, REGION AND PATHOLOGY
    BAYLISS, MT
    JOHNSTONE, B
    OBRIEN, JP
    [J]. SPINE, 1988, 13 (09) : 972 - 981
  • [2] CHYMOPAPAIN, CHEMONUCLEOLYSIS, AND NUCLEUS PULPOSUS REGENERATION
    BRADFORD, DS
    COOPER, KM
    OEGEMA, TR
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1983, 65 (09) : 1220 - 1231
  • [3] CHYMOPAPAIN, CHEMONUCLEOLYSIS, AND NUCLEUS PULPOSUS REGENERATION - A BIOCHEMICAL AND BIOMECHANICAL STUDY
    BRADFORD, DS
    OEGEMA, TR
    COOPER, KM
    WAKANO, K
    CHAO, EY
    [J]. SPINE, 1984, 9 (02) : 135 - 147
  • [4] MYOGENIC SPECIFICATION OF SOMITES IS MEDIATED BY DIFFUSIBLE FACTORS
    BUFFINGER, N
    STOCKDALE, FE
    [J]. DEVELOPMENTAL BIOLOGY, 1995, 169 (01) : 96 - 108
  • [5] DISKS DEGENERATE BEFORE FACETS
    BUTLER, D
    TRAFIMOW, JH
    ANDERSSON, GBJ
    MCNEILL, TW
    HUCKMAN, MS
    [J]. SPINE, 1990, 15 (02) : 111 - 113
  • [6] A new culture system to study the metabolism of the intervertebral disc in vitro
    Chiba, K
    Andersson, GBJ
    Masuda, K
    Momohara, S
    Williams, JM
    Thonar, EJMA
    [J]. SPINE, 1998, 23 (17) : 1821 - 1827
  • [7] Metabolism of the extracellular matrix formed by intervertebral disc cells cultured in alginate
    Chiba, K
    Andersson, GBJ
    Masuda, K
    Thonar, EJMA
    [J]. SPINE, 1997, 22 (24) : 2885 - 2893
  • [8] THE PROTEOGLYCANS OF THE CANINE INTERVERTEBRAL-DISK
    COLE, TC
    BURKHARDT, D
    FROST, L
    GHOSH, P
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 839 (02) : 127 - 138
  • [9] COMPER WD, 1996, EXTRACELLULAR MATRIX, V2, P1
  • [10] Ghosh, 1988, BIOL INTERVERTEBRAL, P39