Increased apoptotic potential and dose-enhancing effect of gold nanoparticles in combination with single-dose clinical electron beams on tumor-bearing mice

被引:215
作者
Chang, Meng-Ya [1 ]
Shiau, Ai-Li [2 ]
Chen, Yu-Hung [1 ]
Chang, Chih-Jui [1 ]
Chen, Helen H-W [3 ,4 ]
Wu, Chao-Liang [1 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Biochem & Mol Biol, Tainan 701, Taiwan
[2] Natl Cheng Kung Univ Hosp, Dept Microbiol & Immunol, Tainan 701, Taiwan
[3] Natl Cheng Kung Univ, Coll Med, Inst Clin Med, Tainan 701, Taiwan
[4] Natl Cheng Kung Univ Hosp, Dept Radiat Oncol, Tainan 701, Taiwan
来源
CANCER SCIENCE | 2008年 / 99卷 / 07期
关键词
D O I
10.1111/j.1349-7006.2008.00827.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
High atomic number material, such as gold, may be used in conjunction with radiation to provide dose enhancement in tumors. In the current study, we investigated the dose-enhancing effect and apoptotic potential of gold nanoparticles in combination with single-dose clinical electron beams on B16F10 melanoma tumor-bearing mice. We revealed that the accumulation of gold nanoparticles was detected inside B16F10 culture cells after 18 h of incubation, and moreover, the gold nanoparticles were shown to be colocalized with endoplasmic reticulum and Golgi apparatus in cells. Furthermore, gold nanoparticles radiosensitized melanoma cells in the colony formation assay (P = 0.02). Using a B16F10 tumor-bearing mouse model, we further demonstrated that gold nanoparticles in conjunction with ionizing radiation significantly retarded tumor growth and prolonged survival compared to the radiation alone controls (P < 0.05). Importantly, an increase of apoptotic signals was detected inside tumors in the combined treatment group (P < 0.05). Knowing that radiation-induced apoptosis has been considered a determinant of tumor responses to radiation therapy, and the length of tumor regrowth delay correlated with the extent of apoptosis after single-dose radiotherapy, these results may suggest the clinical potential of gold nanoparticles in improving the outcome of melanoma radiotherapy.
引用
收藏
页码:1479 / 1484
页数:6
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