Decay of Bacteroidales Genetic Markers in Relation to Traditional Fecal Indicators for Water Quality Modeling of Drinking Water Sources

被引:46
作者
Sokolova, Ekaterina [1 ]
Astrom, Johan [1 ]
Pettersson, Thomas J. R. [1 ]
Bergstedt, Olof [2 ]
Hermansson, Malte [3 ]
机构
[1] Chalmers Univ Technol, SE-41296 Gothenburg, Sweden
[2] City Gothenburg, Gothenburg Water, SE-42423 Angered, Sweden
[3] Univ Gothenburg, Dept Cell & Mol Biol, SE-40530 Gothenburg, Sweden
关键词
REAL-TIME PCR; ESCHERICHIA-COLI; MORTALITY-RATES; RIBOSOMAL DNA; SPRING WATER; FRESH-WATER; SURVIVAL; RNA; PERSISTENCE; TEMPERATURE;
D O I
10.1021/es2024498
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The implementation of microbial fecal source tracking (MST) methods in drinking water management is limited by the lack of knowledge on the transport and decay of host-specific genetic markers in water sources. To address these limitations, the decay and transport of human (BacH) and ruminant (BacR) fecal Bacteroidales 16S rRNA genetic markers in a drinking water source (Lake Radasjon in Sweden) were simulated using a microbiological model coupled to a three-dimensional hydrodynamic model. The microbiological model was calibrated using data from outdoor microcosm trials performed in March, August, and November 2010 to determine the decay of BacH and BacR markers in relation to traditional fecal indicators. The microcosm trials indicated that the persistence of BacH and BacR in the microcosms was not significantly different from the persistence of traditional fecal indicators. The modeling of BacH and BacR transport within the lake illustrated that the highest levels of genetic markers at the raw water intakes were associated with human fecal sources (on-site sewers and emergency sewer overflow). This novel modeling approach improves the interpretation of MST data, especially, when fecal pollution from the same host group is released into the water source from different sites in the catchment.
引用
收藏
页码:892 / 900
页数:9
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