Using PCR for unraveling the cryptic epizootiology of livestock trypanosomosis in the Pantanal, Brazil

被引:58
作者
Dávila, AMR
Herrera, HM
Schlebinger, T
Souza, SS
Traub-Cseko, YM
机构
[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Mol Tripanosomatideos, Dept Bioquim & Biol Mol, BR-21045900 Rio De Janeiro, Brazil
[2] Fiocruz MS, Inst Oswaldo Cruz, Dept Protozool, Lab Biol Tripanosomatideos, BR-21045900 Rio De Janeiro, Brazil
关键词
Trypanosoma vivax; Trypanosoma evansi; PCR; diagnosis; protozoa; livestock; pantanal; Brazil;
D O I
10.1016/j.vetpar.2003.08.002
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Trypanosoma vivax and Trypanosoma evansi are livestock parasites of economic importance in Africa, Asia and South America. In the Pantanal, Brazil, they cause economic losses in both cattle and equines. Little is known of their maintenance and spread in nature, particularly in terms of reservoirs and means of mechanical transmission. Here we report for the first time the use of PCR for the detection of T vivax and T evansi in bovines, buffaloes and sheep. Whereas parasitological diagnosis detected only two T vivax infections, one in buffalo and another in a cow, PCR detected infections in 34.8% buffaloes, 44.7% bovines and 37.3% sheep. Trypanozoon primers detected 41.8% infections in buffaloes and 8.1% in cattle. PCR revealed 6.9% mixed infections in buffaloes and 5.3% in cattle. The potential role of cattle and buffaloes as hosts and reservoirs of T vivax is discussed, as well as the implications of possible extravascular foci in the maintenance of livestock trypanosomosis. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:1 / 13
页数:13
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