Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1

被引：26

作者：

Fujita, T ^{[1
]}

Ikuta, J ^{[1
]}

Hamada, J ^{[1
]}

Okajima, T ^{[1
]}

Tatematsu, K ^{[1
]}

Tanizawa, K ^{[1
]}

Kuroda, S ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 313卷 / 03期

关键词：

FEZ1; FEZ2; UNC-76; axonal guidance; protein kinase C zeta;

D O I：

10.1016/j.bbrc.2003.12.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fasciculation and elongation protein zeta-1 (FEZ I) is a mammalian orthologue of the Caenorhabditis elegans UNC-76 protein involved in the axonal outgrowth and fasciculation and promotes neurite extension of PC 12 cells through interaction with protein kinase C zeta (PKCzeta). The gene coding for FEZ2, a homologue of FEZ I, has also been reported in rat and human. In this study, we compared mRNA expression of FEZ1 and FEZ2 in adult rat tissues and mouse embryos by Northern blot and in situ hybridization analyses. In contrast to FEZ1 whose mRNA is expressed almost exclusively in rat brain and temporarily around the neurogenesis stage of mouse embryos, the message for FEZ2 is detected weakly in most tissues and abundantly throughout the mouse embryonic stages. Similar to FEZ1, FEZ2 interacted with PKCzeta and induced neurite extension of PC12 cells when coexpressed with a constitutively active mutant of PKCzeta. These results suggest that FEZ2 plays an important role in the morphological changes of various cells by associating with PKCzeta in a tissue-non-specific manner. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：738 / 744

页数：7

共 11 条

[1] The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation [J].