PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species:: Application to isolates and stool samples

被引：30

作者：

Metherell, LA ^{[1
]}

Logan, JMJ ^{[1
]}

Stanley, J ^{[1
]}

机构：

[1] Cent Publ Hlth Lab, Mol Biol Unit, Div Virus Reference, London NW9 5HT, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 02期

关键词：

D O I：

10.1128/JCM.37.2.433-435.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report a PCR-enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRnA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA.

引用

页码：433 / 435

页数：3

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