Comparison of some centrifugal partition chromatography systems for a general separation of plant extracts

被引：12

作者：

Ingkaninan, K ^{[1
]}

Hermans-Lokkerbol, ACJ ^{[1
]}

Verpoorte, R ^{[1
]}

机构：

[1] Leiden Univ, Gorlaeus Labs, Leiden Amsterdam Ctr Drug Res, Div Pharmacognosy, NL-2300 RA Leiden, Netherlands

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1999年 / 22卷 / 06期

关键词：

D O I：

10.1081/JLC-100101705

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to find a Centrifugal Partition Chromatography (CPC) solvent system that can be used as a general prefractionation step for crude plant extracts, twelve two-phase systems were compared. The butanol: ethyl acetate: water 3:2:5 (v/v/v) system gave good separation for polar compounds, and the heptane: ethyl acetate: methanol: water 6:1:6:1 (v/v/v/v) system was found to be effective for the separation of nonpolar compounds and somewhat less for polar compounds.

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页码：885 / 896

页数：12

相关论文

共 16 条

[1] CENTRIFUGAL PARTITION CHROMATOGRAPHY .4. PREPARATIVE SAMPLE PURIFICATION AND PARTITION-COEFFICIENT DETERMINATION [J].

BERTHOD, A ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (06) :1187-1204

[2] CENTRIFUGAL PARTITION CHROMATOGRAPHY .6. TEMPERATURE EFFECTS [J].

BERTHOD, A ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (07) :1457-1474

[3] CENTRIFUGAL PARTITION CHROMATOGRAPHY .2. SELECTIVITY AND EFFICIENCY [J].

BERTHOD, A ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (03) :567-583

[4] CENTRIFUGAL PARTITION CHROMATOGRAPHY .1. GENERAL FEATURES [J].

BERTHOD, A ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (03) :547-566

[5] CENTRIFUGAL PARTITION CHROMATOGRAPHY .5. OCTANOL-WATER PARTITION-COEFFICIENTS, DIRECT AND INDIRECT DETERMINATION [J].

BERTHOD, A ;

HAN, YI ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (07) :1441-1456

[6] CENTRIFUGAL PARTITION CHROMATOGRAPHY .3. PHYSICOCHEMICAL PROPERTIES OF TERNARY LIQUID-SYSTEMS [J].

BERTHOD, A ;

DUNCAN, JD ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (06) :1171-1185

[7] GRADIENT ELUTION CENTRIFUGAL PARTITION CHROMATOGRAPHY - COMPARISON WITH HPLC GRADIENTS AND USE OF TERNARY DIAGRAMS TO BUILD GRADIENTS [J].

FOUCAULT, A ;

NAKANISHI, K .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1990, 13 (18) :3583-3602

[8]

FOUCAULT A, 1994, CENTRIFUGAL PARTITIO, P363

[9]

FOUCAULT A, 1994, CENTRIFUGAL PARTITIO, P71

[10] BIPHASIC MIXTURE OF WATER, DIMETHYL-SULFOXIDE, AND TETRAHYDROFURAN FOR USE IN CENTRIFUGAL PARTITION CHROMATOGRAPHY [J].

FOUCAULT, AP ;

DURAND, P ;

FRIAS, EC ;

LEGOFFIC, F .

ANALYTICAL CHEMISTRY, 1993, 65 (15) :2150-2154