3D characterization of natural colloids by FIFFF-MALLS-TEM

被引：39

作者：

Baalousha, M

Kammer, FVD

Motelica-Heino, M

Le Coustumer, P

机构：

[1] Univ Bordeaux 1, Ctr Dev Appl Geol, F-33400 Talence, France

[2] Tech Univ Hamburg, Dept Environm Sci & Technol, D-21073 Hamburg, Germany

[3] Bur Rech Geol & Minieres, F-45060 Orleans, France

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2005年 / 383卷 / 04期

关键词：

FIFFF; MALLS; TEM; natural colloids; 3D characterization;

D O I：

10.1007/s00216-005-0006-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding the role of colloids in the environment needs more complete characterization of their size and shape than the hydrodynamic diameter usually used. Novel methodology for three-dimensional description of colloids has been successfully established in this work by coupling flow-field flow fractionation (FIFFF) to multi-angle laser-light scattering (MALLS) and transmission electron microscopy (TEM). The methodology was evaluated for a soil colloid sample. Results showed that particle surface areas determined by FIFFF-MALLS-TEM are 3-4 times greater than those calculated using the hydrodynamic diameter determined by FIFFF. Further, results indicated that surface area/volume values are 3-5 times greater than those calculated on the basis of the hydrodynamic diameter. A correction factor must therefore be applied when particle surface area is calculated from the hydrodynamic diameter determined by FIFFF.

引用

页码：549 / 556

页数：8

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