Rapid fluorescence enzyme-linked immunosorbent assay for dexamethasone in equine urine

被引：4

作者：

Hassan, SS ^{[1
]}

Rowell, FJ ^{[1
]}

Jackson, LS ^{[1
]}

机构：

[1] HORSE RACING FORENS LAB LTD,NEWMARKET CB8 7DT,SUFFOLK,ENGLAND

来源：

ANALYTICAL COMMUNICATIONS | 1996年 / 33卷 / 09期

关键词：

D O I：

10.1039/ac9963300299

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive competitive ELISA with a fluorescent end-point has been developed for the measurement of dexamethasone in equine urine samples. Use of pre-mixed reagent of untreated sheep antiserum and alkaline phosphatase-labelled second antibody enables the analysis of 40 duplicate urine samples within 55 min. The assay has good precision (RSD less than 10% for within and between assay runs) and is reasonably sensitive (LOD equivalent to 174 ng ml(-1) of diluted equine sample). The assay has been used to detect the drug over 23 h in urine from a race horse following intramuscular injection of 20 mg of dexamethasone sodium phosphate.

引用

页码：299 / 302

页数：4

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