Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins

被引：162

作者：

Koehler, CM ^{[1
]}

Merchant, S ^{[1
]}

Oppliger, W ^{[1
]}

Schmid, K ^{[1
]}

Jarosch, E ^{[1
]}

Dolfini, L ^{[1
]}

Junne, T ^{[1
]}

Schatz, G ^{[1
]}

Tokatlidis, K ^{[1
]}

机构：

[1] Univ Basel, Bioctr, CH-4056 Basel, Switzerland

来源：

EMBO JOURNAL | 1998年 / 17卷 / 22期

关键词：

extragenic suppression; mitochondrial protein import; Saccharomyces cerevisiae; TIM complex;

D O I：

10.1093/emboj/17.22.6477

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tim10p, a protein of the yeast mitochondrial intermembrane space, was shown previously to be essential for the import of multispanning carrier proteins from the cytoplasm into the inner membrane. We now identify Tim9p, another essential component of this import pathway. Most of Tim9p is associated with Tim10p in a soluble 70 kDa complex. Tim9p and Tim10p copurify in successive chromatographic fractionations and co-immunoprecipitated with each other, Tim9p can be cross-linked to a partly translocated carrier protein, A small fraction of Tim9p is bound to the outer face of the inner membrane in a 300 kDa complex whose other subunits include Tim54p, Tim22p, Tim12p and Tim10p, The sequence of Tim9p is 25% identical to that of Tim10p and Tim12p, A Ser(67)-->Cys(67) mutation in Tim9p suppresses the temperature-sensitive growth defect of tim10-1 and tim12-1 mutants. Tim9p is a new subunit of the TIM machinery that guides hydrophobic inner membrane proteins across the aqueous intermembrane space.

引用

页码：6477 / 6486

页数：10

共 41 条

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