Interference of carboxy-PTIO with nitric oxide- and peroxynitrite-mediated reactions

被引:79
作者
Pfeiffer, S
Leopold, E
Hemmens, B
Schmidt, K
Werner, ER
Mayer, B
机构
[1] GRAZ UNIV, INST PHARMAKOL & TOXIKOL, A-8010 GRAZ, AUSTRIA
[2] UNIV INNSBRUCK, INST MED CHEM & BIOCHEM, A-6020 INNSBRUCK, AUSTRIA
基金
奥地利科学基金会;
关键词
carboxy-PTIO; nitric oxide; 3-nitrotyrosine; peroxynitrite; SIN-1; S-nitrosoglutathione;
D O I
10.1016/S0891-5849(96)00407-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Carboxy-PTIO reacts rapidly with NO to yield NO2 and has been used as a scavenger to test the importance of nitric oxide (NO) in various physiological conditions. This study investigated the effects of carboxy-PTIO on several NO- and peroxynitrite-mediated reactions. The scavenger potently inhibited NO-induced accumulation of cGMP in endothelial cells but potentiated the effect of the putative peroxynitrite donor SIN-1. Carboxy-PTIO completely inhibited peroxynitrite-induced formation of 3-nitrotyrosine from free tyrosine (EC(50) = 36 +/- 5 mu M) as well as nitration of bovine serum albumin. Peroxynitrite-mediated nitrosation of GSH was stimulated by the drug with an EC(50) of 0.12 +/- 0.03 mM, whereas S nitrosation induced by the NO donor DEA/NO (0.1 mM) was inhibited by the scavenger with an IC50 of 0.11 +/- 0.03 mM. Oxidation of NO with carboxy-PTIO resulted in formation of nitrite without concomitant production of nitrate. Our results demonstrate that the effects of carboxy-PTIO are diverse and question its claimed specificity as NO scavenger. Copyright (C) 1997 by Elsevier Science Inc.
引用
收藏
页码:787 / 794
页数:8
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