Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomics

被引：82

作者：

Jungblut, PR

Müller, EC

Mattow, J

Kaufmann, SHE

机构：

[1] Max Planck Inst Infect Biol, Core Facil Prot Anal, D-10117 Berlin, Germany

[2] Max Planck Inst Infect Biol, Dept Immunol, D-10117 Berlin, Germany

[3] Max Delbruck Ctr Mol Med, Berlin, Germany

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 09期

关键词：

D O I：

10.1128/IAI.69.9.5905-5907.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser desorption ionization and nano-electrospray mass spectrometry.

引用

页码：5905 / 5907

页数：3

共 13 条

[1] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[2] Analysis of changes in acute-phase plasma proteins in an acute inflammatory response and in rheumatoid arthritis using two-dimensional gel electrophoresis [J].

Doherty, NS ;

Littman, BH ;

Reilly, K ;

Swindell, AC ;

Buss, JM ;

Anderson, NL .

ELECTROPHORESIS, 1998, 19 (02) :355-363

[3] WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].

FLEISCHMANN, RD ;

ADAMS, MD ;

WHITE, O ;

CLAYTON, RA ;

KIRKNESS, EF ;

KERLAVAGE, AR ;

BULT, CJ ;

TOMB, JF ;

DOUGHERTY, BA ;

MERRICK, JM ;

MCKENNEY, K ;

SUTTON, G ;

FITZHUGH, W ;

FIELDS, C ;

GOCAYNE, JD ;

SCOTT, J ;

SHIRLEY, R ;

LIU, LI ;

GLODEK, A ;

KELLEY, JM ;

WEIDMAN, JF ;

PHILLIPS, CA ;

SPRIGGS, T ;

HEDBLOM, E ;

COTTON, MD ;

UTTERBACK, TR ;

HANNA, MC ;

NGUYEN, DT ;

SAUDEK, DM ;

BRANDON, RC ;

FINE, LD ;

FRITCHMAN, JL ;

FUHRMANN, JL ;

GEOGHAGEN, NSM ;

GNEHM, CL ;

MCDONALD, LA ;

SMALL, KV ;

FRASER, CM ;

SMITH, HO ;

VENTER, JC .

SCIENCE, 1995, 269 (5223) :496-512

[4] Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains:: towards functional genomics of microbial pathogens [J].

Jungblut, PR ;

Schaible, UE ;

Mollenkopf, HJ ;

Zimny-Arndt, U ;

Raupach, B ;

Mattow, J ;

Halada, P ;

Lamer, S ;

Hagens, K ;

Kaufmann, SHE .

MOLECULAR MICROBIOLOGY, 1999, 33 (06) :1103-1117

[5] Is the development of a new tuberculosis vaccine possible? [J].

Kaufmann, SHE .

NATURE MEDICINE, 2000, 6 (09) :955-960

[6] 2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS - AN UPDATED PROTOCOL AND IMPLICATIONS FOR A FUNCTIONAL-ANALYSIS OF THE GENOME [J].

KLOSE, J ;

KOBALZ, U .

ELECTROPHORESIS, 1995, 16 (06) :1034-1059

[7] Matrix-assisted laser desorption-ionization mass spectrometry peptide mass fingerprinting for proteome analysis: identification efficiency after on-blot or in-gel digestion with and without desalting procedures [J].

Lamer, S ;

Jungblut, PR .

JOURNAL OF CHROMATOGRAPHY B, 2001, 752 (02) :311-322

[8] ERROR TOLERANT IDENTIFICATION OF PEPTIDES IN SEQUENCE DATABASES BY PEPTIDE SEQUENCE TAGS [J].

MANN, M ;

WILM, M .

ANALYTICAL CHEMISTRY, 1994, 66 (24) :4390-4399

[9]

Mattow J, 2001, PROTEOMICS, V1, P494, DOI 10.1002/1615-9861(200104)1:4<494::AID-PROT494>3.0.CO

[10]

2-4

← 1 2 →