Cell-autonomous complementation of mlo resistance using a biolistic transient expression system

被引:93
作者
Shirasu, K
Nielsen, K
Piffanelli, P
Oliver, R
Schulze-Lefert, P
机构
[1] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[2] Carlsberg Lab, Dept Physiol, DK-2500 Copenhagen, Denmark
关键词
D O I
10.1046/j.1365-313X.1999.00376.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The barley gene Mlo encodes a prototype of a novel class of plant proteins. In mlo mutants, absence of the 60 kDa wild-type Mlo protein results in broad-spectrum resistance to the powdery mildew fungus, Erysiphe graminis f, sp. hordei. To directly assess its function, Mlo was transiently expressed with a marker gene encoding a modified green fluorescent protein (GFP) in leaf epidermal cells of mlo resistant barley lines. Fungal inoculation of epidermal cells transfected with wild-type Mlo led to haustorium formation and abundant sporulation. Therefore, expression of the wild-type Mlo gene, in mlo resistant genotypes, is both necessary and sufficient to restore susceptibility to fungal attack. Complementation of mlo resistance alleles was restricted to single host cells, indicating a cell-autonomous function for the wild-type Mlo protein. We discuss our findings with respect to source-sink relationships of plants and biotrophic fungi and the potentially wide-ranging use of the transient complementation assay to analyse host compatibility and defence in response to powdery mildew attack.
引用
收藏
页码:293 / 299
页数:7
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