Using GFP in FRET-based applications

被引：372

作者：

Pollok, BA ^{[1
]}

Heim, R ^{[1
]}

机构：

[1] Aurora Biosci Corp, San Diego, CA 92121 USA

来源：

TRENDS IN CELL BIOLOGY | 1999年 / 9卷 / 02期

关键词：

D O I：

10.1016/S0962-8924(98)01434-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The use of green fluorescent protein (GFP) is a powerful technology that has recently enabled investigators to study dynamic molecular events within living cells. One method for detecting molecular interactions involves fluorescence resonance energy transfer (FRET) between two GFPs or between GFP and a second fluorophore. This review summarizes the use of GFP for FRET and illustrates the theme with specific examples on how GFP has been employed as an intracellular molecular sensor.

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页码：57 / 60

页数：4

相关论文

共 14 条

[1]

Cubitt AB, 1999, METHOD CELL BIOL, V58, P19

[2] Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy [J].

Day, RN .

MOLECULAR ENDOCRINOLOGY, 1998, 12 (09) :1410-1419

[3] Specific covalent labeling of recombinant protein molecules inside live cells [J].

Griffin, BA ;

Adams, SR ;

Tsien, RY .

SCIENCE, 1998, 281 (5374) :269-272

[4]

HEIM R, 1996, CURR BIOL, V6, P1178

[5] Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins [J].

Llopis, J ;

McCaffery, JM ;

Miyawaki, A ;

Farquhar, MG ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (12) :6803-6808

[6] Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer [J].

Mahajan, NP ;

Linder, K ;

Berry, G ;

Gordon, GW ;

Heim, R ;

Herman, B .

NATURE BIOTECHNOLOGY, 1998, 16 (06) :547-552

[7] Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein [J].

Mitra, RD ;

Silva, CM ;

Youvan, DC .

GENE, 1996, 173 (01) :13-17

[8] Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin [J].

Miyawaki, A ;

Llopis, J ;

Heim, R ;

McCaffery, JM ;

Adams, JA ;

Ikura, M ;

Tsien, RY .

NATURE, 1997, 388 (6645) :882-887

[9]

Ormo M, 1996, SCIENCE, V273, P1392, DOI 10.1126/science.273.5280.1392

[10] Detection in living cells of Ca2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence - A new class of fluorescent indicators [J].

Romoser, VA ;

Hinkle, PM ;

Persechini, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (20) :13270-13274