Variations in the molecular masses of the capsular exopolysaccharides amylovoran, pyrifolan and stewartan

被引：9

作者：

Schollmeyer, Martin ^{[2
]}

Langlotz, Christine ^{[2
]}

Huber, Anton ^{[3
]}

Coplin, David L. ^{[4
]}

Geider, Klaus ^{[1
,2
]}

机构：

[1] Julius Kuhn Inst, Inst Pflanzenschutz Obst & Weinbau, Dossenheim, Germany

[2] Max Planck Inst Cell Biol, Ladenburg, Germany

[3] KF Univ Graz, Inst Chem, CePoL MC Cent Polymer Lab Mol Characterist, EPNOE European Polysaccharide Network Excellence, A-8010 Graz, Austria

[4] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 50卷 / 03期

关键词：

Erwinia amylovora; Fire blight; Asian pear blight; Size of EPS; Stewart's wilt; ERWINIA-AMYLOVORA; ESCHERICHIA-COLI; PEAR TREES; PATHOGEN; BIOSYNTHESIS; PROTEIN; WEIGHT; GENE; LIPOPOLYSACCHARIDE; DIFFERENTIATION;

D O I：

10.1016/j.ijbiomac.2012.01.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Erwinia amylovora, causing fire blight of apple, pear and some ornamentals, Erwinia pyrifoliae, causing Asian pear blight, and Pantoea stewartii, causing Stewart's wilt of sweet maize, synthesize capsular extracellular polysaccharides (EPSs) with a high molecular mass. The EPSs are virulence factors and form viscous aggregates, which participate in clogging vessels of infected plants and causing wilting. The sizes of EPSs produced under different environmental growth conditions were determined by analysis with large pore HPLC columns. Their molecular mass of ca. 5 MDa, when isolated from agar plates, decreases to ca. 1 MDa for E. amylovora amylovoran from freeze-dried supernatants from liquid cultures and to 2 MDa for freeze-dried preparations of P. stewartii stewartan. size changes were also found following growth in various other media and for different strains. Stewartan, amylovoran and E. pyrifoliae pyrifolan were also shown to be completely degraded by a bacteriophage EPS depolymerase. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：518 / 522

页数：5

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