Characterization of RNase P holoenzymes from Methanococcus jannaschii and Methanothermobacter thermoautotrophicus

被引：23

作者：

Andrews, AJ ^{[1
]}

Hall, TA ^{[1
]}

Brown, JW ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Microbiol, Raleigh, NC 27695 USA

来源：

BIOLOGICAL CHEMISTRY | 2001年 / 382卷 / 08期

关键词：

Archaea; Archaebacteria; Methanobacterium; purification; ribonuclease P; ribozyme;

D O I：

10.1515/BC.2001.147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The partial purification and basic biochemical characterization of the RNase P holoenzymes of two species of methanogenic Archaea, Methanothermobacter thermoautotrophicus(previously Methanobacterium thermoautotrophicum strain DeltaH) and Methanococcus jannaschii, are described. The properties of these enzymes, particularly buoyant density in Cs2SO4 and recent information about the subunit composition of the archaeal enzymes, suggest that RNase P enzymes in Archaea are much more alike than earlier studies in Sulfolobus acidocaldarius and Haloferax volcanii suggested.

引用

页码：1171 / 1177

页数：7

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