The dendritic cell-derived protein DC-STAMP is highly conserved and localizes to the endoplasmic reticulum

被引：33

作者：

Eleveld-Trancikova, D ^{[1
]}

Triantis, V ^{[1
]}

Moulin, V ^{[1
]}

Looman, MWG ^{[1
]}

Wijers, M ^{[1
]}

Fransen, JAM ^{[1
]}

Lemckert, AAC ^{[1
]}

Havenga, MJE ^{[1
]}

Figdor, CG ^{[1
]}

Janssen, RAJ ^{[1
]}

Adema, GJ ^{[1
]}

机构：

[1] Univ Nijmegen, Nijmegen Ctr Mol Life Sci, Dept Cell Biol, NL-6500 HB Nijmegen, Netherlands

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 2005年 / 77卷 / 03期

关键词：

mouse; human; APC; CLSM; immunobiology;

D O I：

10.1189/jlb.0804441

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recently, we described the molecular identification of dendritic cell-specific TrAnsMembrane protein (DC-STAMP), a multimembrane-spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC-STAMP (mDC-STAMP) as well as the characterization of the DC-STAMP protein. The results demonstrate that mDC-STAMP is over 90% homologous to hDC-STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC-STAMP expression is enhanced by interleukin-4 and down-regulated upon DC maturation. Analysis of differently tagged DC-STAMP proteins further demonstrates that hDC-STAMP and mDC-STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC-STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC-STAMP-green fluorescent protein fusion protein. These data imply that DC-STAMP may exert its effect in the ER.

引用

页码：337 / 343

页数：7

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