Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin

被引：73

作者：

Allen, AG

Bolitho, S

Lindsay, H

Khan, S

Bryant, C

Norton, P

Ward, P

Leigh, J

Morgan, J

Riches, H

Eastty, S

Maskell, D

机构：

[1] Univ Cambridge, Dept Clin Vet Med, Ctr Vet Sci, Cambridge CB3 0ES, England

[2] Inst Anim Hlth, Newbury RG20 7NN, Berks, England

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 04期

关键词：

D O I：

10.1128/IAI.69.4.2732-2735.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A defined allelic-replacement mutant of the sly gene, encoding a thiol-activated cytolysin, from a European isolate of Streptococcus suis serotype 2 was generated and characterized. Unlike the parental strain, it is nonhemolytic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemic infection.

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页码：2732 / 2735

页数：4

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