The effect of conduction block on the spinal release of immunoreactive-neuropeptide Y (ir-NPY) in the neuropathic rat

被引:6
作者
Colvin, LA [1 ]
Duggan, AW
机构
[1] Univ Edinburgh, Royal Sch Vet Studies, Dept Preclin Vet Sci, Edinburgh EH9 1QH, Midlothian, Scotland
[2] Univ Sydney, Royal N Shore Hosp, St Leonards, NSW 2065, Australia
[3] Univ Edinburgh, Royal Infirm, Dept Anaesthet, Edinburgh EH3 9YW, Midlothian, Scotland
基金
英国惠康基金;
关键词
chronic constriction injury; neuropeptide Y; neuropathic pain; spinal cord; antibody microprobe;
D O I
10.1016/S0304-3959(00)00438-3
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Peripheral nerve injury may result in significant changes in neuropeptide production and the development of neuropathic pain behaviour. Rats with a chronic constriction injury of one sciatic nerve were used to study the spinal release of immunoreactive neuropeptide Y (ir-NPY), using the antibody-coated microprobe technique. Previous work has shown an increase in NPY synthesis by large to medium-sized primary afferent neurones, as well as a new area of ir-NPY release in the deep dorsal horn on the side of nerve injury, when compared to uninjured rats. The stimulus for spontaneous ir-NPY release was unclear, but may have been due to ectopic neuronal discharges developing after nerve injury. This study used local anaesthetic to block all electrical input from the injured nerve. No change was found in the new zone of spontaneous release of ir-NPY in the deep dorsal horn ipsilateral to nerve injury. It appears therefore, that ir-NPY is released from the central termination of primary afferent neurones, without regulation from neuronal activity in the primary afferent neurones themselves. (C) 2001 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:235 / 240
页数:6
相关论文
共 38 条
[1]   FURTHER EVIDENCE FOR PAIN-RELATED BEHAVIORS IN A MODEL OF UNILATERAL PERIPHERAL MONONEUROPATHY [J].
ATTAL, N ;
JAZAT, F ;
KAYSER, V ;
GUILBAUD, G .
PAIN, 1990, 41 (02) :235-251
[2]   PEPTIDE SECRETION - WHAT DO WE KNOW [J].
BEAN, AJ ;
ZHANG, X ;
HOKFELT, T .
FASEB JOURNAL, 1994, 8 (09) :630-638
[3]   A PERIPHERAL MONONEUROPATHY IN RAT THAT PRODUCES DISORDERS OF PAIN SENSATION LIKE THOSE SEEN IN MAN [J].
BENNETT, GJ ;
XIE, YK .
PAIN, 1988, 33 (01) :87-107
[4]   THE EFFECT OF INTRAVENOUS LIDOCAINE, TOCAINIDE, AND MEXILETINE ON SPONTANEOUSLY ACTIVE FIBERS ORIGINATING IN RAT SCIATIC NEUROMAS [J].
CHABAL, C ;
RUSSELL, LC ;
BURCHIEL, KJ .
PAIN, 1989, 38 (03) :333-338
[5]   PATHWAYS TO REGULATED EXOCYTOSIS IN NEURONS [J].
DECAMILLI, P ;
JAHN, R .
ANNUAL REVIEW OF PHYSIOLOGY, 1990, 52 :625-645
[6]   SYSTEMIC LIDOCAINE SILENCES ECTOPIC NEUROMA AND DRG DISCHARGE WITHOUT BLOCKING NERVE-CONDUCTION [J].
DEVOR, M ;
WALL, PD ;
CATALAN, N .
PAIN, 1992, 48 (02) :261-268
[7]   LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC ANALYSIS OF NEUROPEPTIDE Y-IMMUNOREACTIVE PROFILES IN THE CAT SPINAL DORSAL HORN [J].
DOYLE, CA ;
MAXWELL, DJ .
NEUROSCIENCE, 1994, 61 (01) :107-121
[8]   THE PREPARATION AND USE OF ANTIBODY MICROPROBES [J].
DUGGAN, AW ;
HENDRY, IA ;
GREEN, JL ;
MORTON, CR ;
HUTCHISON, WD .
JOURNAL OF NEUROSCIENCE METHODS, 1988, 23 (03) :241-247
[9]  
DUGGAN AW, 1991, MONITORING NEURONAL, P181
[10]  
FIELDS HL, 1994, TXB PAIN, V3, P991