Combination of morphological feature analysis and immunohistochemistry is useful for screening of EML4-ALK-positive lung adenocarcinoma

被引：113

作者：

Jokoji, Ryu ^{[1
]}

Yamasaki, Takashi ^{[1
]}

Minami, Seigo ^{[2
]}

Komuta, Kiyoshi ^{[2
]}

Sakamaki, Yasushi ^{[3
]}

Takeuchi, Kengo ^{[4
]}

Tsujimoto, Masahiko ^{[1
]}

机构：

[1] Osaka Police Hosp, Dept Pathol, Tennouji Ku, Osaka 5430035, Japan

[2] Osaka Police Hosp, Dept Resp Med, Tennouji Ku, Osaka 5430035, Japan

[3] Osaka Police Hosp, Dept Chest Surg, Tennouji Ku, Osaka 5430035, Japan

[4] Japanese Fdn Canc Res, Inst Canc, Div Pathol, Koto Ku, Tokyo 170, Japan

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2010年 / 63卷 / 12期

关键词：

GROWTH-FACTOR RECEPTOR; EML4-ALK FUSION GENE; CLINICOPATHOLOGICAL FEATURES; HISTOLOGICAL CHARACTERISTICS; CANCER; MUTATIONS; GEFITINIB; SYSTEM; EGFR; RARE;

D O I：

10.1136/jcp.2010.081166

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Background A subset of lung cancers harbours the fusion gene echinoderm microtubule-associated protein-like-4-anaplastic lymphoma kinase (EML4-ALK). Recently, immunohistochemistry for ALK has shown sensitivity for the detection of EML4-ALK-positive lung adenocarcinoma almost equal to that of the fluorescence in situ hybridisation (FISH) assay. Aims To study the clinicopathological features of EML4-ALK-positive lung adenocarcinoma in a large number of surgically resected samples using immunohistochemistry, in order to establish a useful screening method for EML4-ALK-positive lung adenocarcinoma. Methods Immunohistochemistry for ALK was used to screen for EML4-ALK-positive lung adenocarcinomas in 254 cases of surgically resected samples. Results EML4-ALK-positive cases were detected in 3.1% of lung adenocarcinomas (8/254). EML4-ALK-positive lung adenocarcinomas showed significant associations with intra- and/or extra-cytoplasmic mucin (p=0.0001), and cribriform pattern with excessive extracytoplasmic mucin (p<0.0001). Signet-ring cell appearance alone lacked significance (p=0.149). Conclusion EML4-ALK-positive lung adenocarcinoma has a tendency to express a characteristic morphological pattern. The combined use of morphological feature analysis and immunohistochemistry may be a useful and cost effective screening method for EML4-ALK lung adenocarcinoma.

引用

页码：1066 / 1070

页数：5

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