Gene arrays are not just for measuring gene expression

被引：33

作者：

Hazen, SP ^{[1
]}

Kay, SA ^{[1
]}

机构：

[1] Scripps Res Inst, La Jolla, CA 92037 USA

来源：

TRENDS IN PLANT SCIENCE | 2003年 / 8卷 / 09期

关键词：

D O I：

10.1016/S1360-1385(03)00186-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Recent advances in the application of high-density oligonucleotide arrays offer new opportunities for gene discovery through genotyping several hundred thousand loci in a single assay. This is accomplished by hybridizing labeled total genomic DNA to a GeneChip rather than using mRNA, which measures gene transcript abundance. The single feature polymorphisms (SFPs) detected by this technique are the consequence of allelic variants ranging from single nucleotide polymorphisms to large deletions that differentially hybridize to the oligonucleotide array features. In yeast, this approach has proven useful in linkage mapping, the dissection of quantitative trait loci, and in assessing species population structure. Recently, the utility of this technology has been shown in the Arabidopsis genome.

引用

页码：413 / 416

页数：4

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