Structure of the replicating complex of a pol α family DNA polymerase

被引：470

作者：

Franklin, MC

Wang, JM

Steitz, TA ^{[1
]}

机构：

[1] Yale Univ, Dept Mol Biophys & Biochem, POB 6666, New Haven, CT 06511 USA

[2] Yale Univ, Dept Chem, New Haven, CT 06511 USA

[3] Yale Univ, Howard Hughes Med Inst, New Haven, CT 06511 USA

来源：

CELL | 2001年 / 105卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(01)00367-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the 2.6 Angstrom resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the ape-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.

引用

页码：657 / 667

页数：11

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