Catalytic reaction of basidiomycete Lentinula edodes cytochrome P450, Le.CYP1 enzyme produced in yeast

被引：3

作者：

Akiyama, R ^{[1
]}

Kajiwara, S ^{[1
]}

Shishido, K ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 01期

关键词：

cytochrome P450; benzo(a)pyrene; 7-ethoxycoumarin; yeast microsome; Lentinula edodes;

D O I：

10.1271/bbb.68.79

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le. CYP1 enzyme. Le. CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.

引用

页码：79 / 84

页数：6

共 19 条

[1]

Adams A., 1997, METHODS YEAST GENETI

[2] Cloning and expression of cytochrome P450 genes, belonging to a new P450 family, of the basidiomycete Lentinula edodes [J].