Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112)

被引：44

作者：

Vinogradov, E

Conlan, WJ

Gunn, JS

Perry, MB ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

[2] Ohio State Univ, Dept Virol Immunol & Med Genet, Dept Med, Div Infect Dis, Columbus, OH 43210 USA

[3] Ohio State Univ, Ctr Microbial Interface Biol, Columbus, OH 43210 USA

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 03期

关键词：

D O I：

10.1016/j.carres.2003.12.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Francisella novicida (U1 12), a close relative of the highly virulent bacterium F tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, H-1 and C-13 NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-D-galacturonamide\(D-GalN-AcAN) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D -Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3: 1) and had the structure: --> 4)-alpha-D-GalNAcAN-(1 --> 4)-alpha-D-GalNAcAN-(1 --> 4)-alpha-D-GalNAcAN-(1 --> 3)-alpha-D-QuiNAc4NAC-(1 --> With polyclonal. murine antibody, the F novicida O-antigen did not show serological. cross-reactivity with the O-antigen of F tularensis despite the occurrence of a common -->4)-D-GalpNAcAN-(1-->4)-alpha-D-GalpNAcAN-(1 --> disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species. Crown Copyright (C) 2003 Published by Elsevier Ltd. All rights reserved.

引用

页码：649 / 654

页数：6

共 18 条

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