Isolation of a novel antimicrobial peptide gene (Sp-AMP) homologue from Pinus sylvestris (Scots pine) following infection with the root rot fungus Heterobasidion annosum

被引:31
作者
Asiegbu, FO
Choi, WB
Li, GS
Nahalkova, J
Dean, RA
机构
[1] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, S-75007 Uppsala, Sweden
[2] N Carolina State Univ, Fungal Genomics Lab, Raleigh, NC 27695 USA
关键词
subtraction hybridization; Heterobasidion annosum; expressed sequence tag; Pinus sylvestris;
D O I
10.1016/S0378-1097(03)00697-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new family of antimicrobial peptide homologues termed Sp-Amp has been discovered in Pinus sylvestris (Scots pine). This is the first report of such proteins to be characterized in a conifer species. Sp-AMP1 was identified in a substructured cDNA library of root tissue infected with the root rot fungus Heterobasidion annosum and encodes a mature peptide of 79 amino acid residues. Three additional members of the Sp-AMP family (Sp-AMPs 2-4) encode cysteine-rich proteins of 105 amino acids, each containing an N-terminal region with a probable cleavage signal sequence. Northern analysis confirmed that Sp-AMP expression is elevated in Scots pine roots upon infection with H. annosum. These peptides share 64% amino acid identity with a mature protein from Macadamia integrifolia (MiAMP1), which allowed us to build a homology model for preliminary analysis. Southern analyses further confirmed that several copies of the gene are present in the Scots pine genome. The potential significance of Sp-AMP in the H. annosum-conifer pathosystem is discussed. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:27 / 31
页数:5
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