Nonaqueous capillary electrophoretic separation of polyphenolic compounds in wine using coated capillaries at high pH in methanol

被引:24
作者
Demianová, Z
Sirén, H
Kuldvee, R
Riekkola, ML
机构
[1] Univ Helsinki, Analyt Chem Lab, Dept Chem, FIN-00014 Helsinki, Finland
[2] Tallinn Univ Technol, Dept Chem, Fac Sci, EE-200108 Tallinn, Estonia
关键词
coated capillary; flavonoid; nonaqueous capillary zone electrophoresis resveratrol; wine;
D O I
10.1002/elps.200305688
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Nonaqueous capillary electrophoretic separation of a group of flavonoids (guercetin, myricetin, catechin, epicatechin) and resveratrol in wine was investigated in methanol at high pH. Malonate background electrolyte (pH* 13.5, ionic strength / = 14.2 mmol/L) provided highly repeatable separations of the analytes. Tests of untreated and coated (poly(glycidylmethacrylate-co-N-vinylpyrrolidone)) capillaries showed the analysis to be faster (6.5 min vs. 25 min) and the repeatability better in the coated capillaries. The coating procedure was simple and highly repeatable and the coating was stable during 40-45 runs. Determination of the last migrating peaks (epicatechin, resveratrol and catechin) was achieved merely by evaporating the wine samples and reconstituting the residue in methanol. For determination of the first migrating peaks (quercetin and myricetin) the samples were submitted to solid-phase extraction in C8 cartridges.
引用
收藏
页码:4264 / 4271
页数:8
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