Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye

被引：55

作者：

Li, CP

Yu, HH

Liu, S

Xing, RE

Guo, ZY

Li, PC ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 24期

关键词：

jellyfish; protease activity; Rhopilema esculentum Kishinouye; venom;

D O I：

10.1016/j.bmcl.2005.09.010

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In this paper, the effects of some chemical and physical factors such as temperature, pH values, glycerol, and divalent metal cations on the protease activity of venom from jellyfish, Rhopilema esculentum Kishinouye, were assayed. Protease activity was dependent on temperature and pH values. Zn2+, Mg2+, and Mn2+ in sodium phosphate buffer (0.02 M, pH 8.0) could increase protease activity. Mn2+ had the best effects among the three metal cations and the effect was about 20 times of that of Zn2+ or Mg2+ and its maximal protease activity was 2.3 x 10(5) U/mL. EDTA could increase protease activity. PMSF had hardly affected protease activity. O-Phenanthroline and glycerol played an important part in inhibiting protease activity and their maximal inhibiting rates were 87.5% and 82.1%, respectively. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：5370 / 5374

页数：5

共 35 条

[1] A functional comparison of the venom of three Australian jellyfish -: Chironex fleckeri, Chiropsalmus sp., and Carybdea xaymacana -: on cytosolic Ca2+, haemolysis and Artemia sp. lethality [J].