The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells

被引：488

作者：

Guo, Fan ^{[1
,2
]}

Yan, Liying ^{[1
,2
,3
]}

Guo, Hongshan ^{[1
,2
]}

Li, Lin ^{[1
,2
]}

Hu, Boqiang ^{[1
,2
]}

Zhao, Yangyu ^{[1
,2
,3
]}

Yong, Jun ^{[1
,2
]}

Hu, Yuqiong ^{[1
,2
,5
,9
]}

Wang, Xiaoye ^{[1
,2
,3
]}

Wei, Yuan ^{[1
,2
,3
]}

Wang, Wei ^{[1
,2
,3
]}

Li, Rong ^{[1
,2
,3
]}

Yan, Jie ^{[1
,2
,3
]}

Zhi, Xu ^{[1
,2
,3
]}

Zhang, Yan ^{[1
,2
,3
]}

Jin, Hongyan ^{[1
,2
,3
]}

Zhang, Wenxin ^{[1
,2
,3
]}

Hou, Yu ^{[1
,2
]}

Zhu, Ping ^{[1
,2
,5
]}

Li, Jingyun ^{[1
,2
,5
]}

Zhang, Ling ^{[6
]}

Liu, Sirui ^{[6
]}

Ren, Yixin ^{[1
,2
,3
]}

Zhu, Xiaohui ^{[1
,2
,3
]}

Wen, Lu ^{[1
,2
,4
]}

Gao, Yi Qin ^{[6
]}

Tang, Fuchou ^{[1
,2
,4
,5
,7
]}

Qiao, Jie ^{[1
,2
,3
,5
,8
]}

机构：

[1] Peking Univ, Hosp 3, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China

[2] Peking Univ, Dept Obstet & Gynecol, Coll Life Sci, Hosp 3, Beijing 100871, Peoples R China

[3] Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China

[4] Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China

[5] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

[6] Peking Univ, Coll Chem & Mol Engn, Inst Theoret & Computat Chem, Beijing 100871, Peoples R China

[7] Peking Univ, Ctr Mol & Translat Med, Hlth Sci Ctr, Beijing 100191, Peoples R China

[8] Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China

[9] Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

来源：

CELL | 2015年 / 161卷 / 06期

基金：

中国国家自然科学基金;

关键词：

EMBRYONIC STEM-CELLS; SINGLE-CELL; GENE-EXPRESSION; DEMETHYLATION; GENOME; DYNAMICS; MICE; SPECIFICATION; PATHWAY; ERASURE;

D O I：

10.1016/j.cell.2015.05.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental-stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10 to 11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation, with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.

引用

页码：1437 / 1452

页数：16

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