Hypothalamic growth hormone-releasing hormone mRNA varies across the day in rats

被引：45

作者：

Bredow, S

Taishi, P

Obel, F

GuhaThakurta, N

Krueger, JM

机构：

[1] UNIV TENNESSEE,CTR HLTH SCI,DEPT PHYSIOL & BIOPHYS,MEMPHIS,TN 38163

[2] ALBERT SZENT GYORGYI MED UNIV,DEPT PHYSIOL,SZEGED,HUNGARY

来源：

NEUROREPORT | 1996年 / 7卷 / 15-17期

关键词：

sleep; RT-PCR; brain; growth hormone-releasing hormone; GHRH; rat; diurnal variation;

D O I：

10.1097/00001756-199611040-00020

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

EXPERIMENTS were performed to determine whether growth hormone-releasing hormone (GHRH) mRNA displays diurnal variations in the hypothalamus and cortex of the rat. Levels of GHRH and beta-actin mRNA were measured from hypothalamic and cortical extracts using the reverse transcriptase polymerase chain reaction method in rats sacrificed at 4 h intervals across a 12:12 h light:dark cycle. Hypothalamic GHRH mRNA peaked around the light onset, declined during the light period, and stayed low in the dark. Variations in hypothalamic beta-actin and cortical GHRH mRNA levels were not observed. beta-Actin mRNA expression in the cortex was higher in the dark than in the light period. The results demonstrate that hypothalamic GHRH mRNA displays diurnal variations.

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页码：2501 / 2505

页数：5

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