Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity

被引：213

作者：

Gao, RM

Yuan, ZB ^{[1
]}

Zhao, ZQ

Gao, XR

机构：

[1] Univ Sci & Technol China, Acad Sinica, Grad Sch, Dept Chem, Beijing 100039, Peoples R China

[2] Hebei Normal Univ, Dept Chem, Shijiazhuang 050016, Peoples R China

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1998年 / 45卷 / 01期

基金：

中国国家自然科学基金;

关键词：

pyrogallol autoxidation; SOD activity; single-sweep oscillopolarography;

D O I：

10.1016/S0302-4598(98)00072-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The autoxidation of pyrogallol was investigated in the presence of EDTA or DETAPAC (diethylenetriaminepentaacetic acid) in the pH range 7.87 to 9.10. Pyrogallol reacts with dioxygen in weakly alkaline solutions to form several intermediate products which are electroactive substance and can be detected by electroanalytical methods. The focus here was putted on the effect of pH on the autoxidation rate of some intermediate products produced in pyrogallol autoxidation, which gives sensitive second-order derivative cathodic waves at -0.20, -0.96 and -1.45 V vs. SCE, respectively. Reaction mechanism was discussed. The paper also presented a convenient electroanalytical assay for superoxide dismutase enzyme activity. (C) 1998 Elsevier Science S.A.

引用

页码：41 / 45

页数：5

共 20 条

[1] A KINETIC-STUDY OF THE AIR OXIDATION OF PYROGALLOL AND PURPUROGALLIN [J].