Triphenyltin acetate toxicity: A biochemical and ultrastructural study on mouse thymocytes

被引:9
作者
Bollo, E [1 ]
Ceppa, L [1 ]
Cornaglia, E [1 ]
Nebbia, C [1 ]
Biolatti, B [1 ]
Dacasto, M [1 ]
机构
[1] UNIV TURIN, SETTORE FARMACOL & TOSSICOL, I-10149 TURIN, ITALY
关键词
mouse; lymphocyte; triphenyltin acetate; apoptosis;
D O I
10.1177/096032719601500306
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
1 Triphenyltin acetate (TPTA) has been shown to exert in vivo a selective toxic effect on the immune system. To assess in vitro possible alterations induced by TPTA exposure, primary cultures of mouse thymocytes were incubated up to 24 h with graded amounts (1 - 12 mu M) of the organotin. 2 The cytotoxic activity has been evaluated with the MTT colorimetric assay, the neutral red (NR) assay and the lactic dehydrogenase (LDH) cellular release. Cell pellets were fixed with 2.5% glutaraldehyde, resin-embedded and ultrathin sections were observed through transmission electron microscopy. 3 After 2 h of incubation, dose-dependent increases of cytotoxicity were observed in thymocytes submitted to MTT and NR tests (up to 41.43% and 18.9%, respectively), while 22 h later this overt effect on cell viability was noticed merely in cells exposed to 12 mu M TPTA. Dose-dependent increases of LDH leakage in the culture medium were observed all throughout the study. 4 Morphological investigations revealed features (chromatin condensation, cell membranes fragmentation and formation of membrane bound apoptotic bodies) suggestive of apoptosis. 5 This study indicates that TPTA is cytotoxic to mouse thymocytes: morphologically, the rising of apoptosis is likely to be recognized, as previously reported in different in vitro studies with other immunosuppressive agents as dioxin and corticosteroids.
引用
收藏
页码:219 / 225
页数:7
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