Evaluation of commercial von Willebrand factor collagen binding assays to assist the discrimination of types 1 and 2 von Willebrand disease

被引：49

作者：

Favaloro, Emmanuel J. ^{[1
]}

机构：

[1] Westmead Hosp, SWAHS, ICPMR, Dept Haematol, Westmead, NSW 2145, Australia

来源：

THROMBOSIS AND HAEMOSTASIS | 2010年 / 104卷 / 05期

关键词：

von Willebrand factor VWF collagen binding; von Willebrand disease; VWD; diagnosis; classification; IDENTIFICATION; DIAGNOSIS; PATHOLOGISTS; EXPERIENCE; THROMBOSIS; GENOTYPE; COLLEGE; UPDATE;

D O I：

10.1160/TH10-06-0360

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study reports on the evaluation of seven commercial von Willebrand factor (VWF) collagen binding (VWF CB) assays to potentially as sist the discrimination of types 1 and 2 von Willebrand disease (VWD) Samples from 25 patients with type 1 VWD of varying severity were cotested with 16 samples from patients with types 2A or 2B VWD plus various control samples using each commercial VWF CB assay assessed against our standard (reference) in house VWF CB assay as well as our in house VWF antigen (VWF Ag) and ristocetin cofactor (VWF RCo) assays Commercial VWF CB assays varied in their ability to discriminate types 1 and 2A/2B VWD The optimal VWF CB/VWF Ag ratio at which optimal discrimination occurred also differed between assays with some improvements observed with some (but not all) as says following a harmonisation process that aimed to correct for different calibrator effects Assay variability also compromised assay utility in some test occasions Future standardisation and improvements in some commercial VWF CB assays are needed before the VWF CB assay can be more fully and globally utilised for discrimination of VWD types in diagnostic laboratories

引用

页码：1009 / 1021

页数：13

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