Simultaneous determination of dermatan sulfate and oversulfated dermatan sulfate in plasma by high-performance liquid chromatography with postcolumn fluorescence derivatization

被引：29

作者：

Huang, Y ^{[1
]}

Washio, Y ^{[1
]}

Hara, M ^{[1
]}

Toyoda, H ^{[1
]}

Koshiishi, I ^{[1
]}

Toida, T ^{[1
]}

Imanari, T ^{[1
]}

机构：

[1] CHIBA UNIV,FAC PHARMACEUT SCI,INAGE KU,CHIBA 263,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1996年 / 240卷 / 02期

关键词：

D O I：

10.1006/abio.1996.0352

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A chemical method for the determination of dermatan sulfate (DS) and oversulfated dermatan sulfate has been developed and applied to the pharmacokinetic study of these polysaccharides in experimental animals. The analytical procedure includes a simple preparation step of administered DS and oversulfated DS from blood plasma, HPLC for the separation and detection of DS and oversulfated DS using an Asahipak NH2P-50 column, fluorometric reaction of the polysaccharides with guanidine in a strong alkaline medium. DS and oversulfated DS were extracted from plasma by treating it with proteinase to remove plasma proteins and recovered with endogenous plasma glycosaminoglycans by ethanol precipitation. Finally, DS and oversulfated DS were analyzed by fluorometric HPLC. The detection limits of DS and oversulfated DS were 10 and 20 ng, respectively. Furthermore, we demonstrated that artificial oversulfation of DS increased its biological half-life after intravenous administration to rats. (C) 1996 Academic Press, Inc.

引用

页码：227 / 234

页数：8

共 30 条

[1] CAPILLARY ELECTROPHORESIS FOR THE ANALYSIS OF CHONDROITIN SULFATE-DERIVED AND DERMATAN SULFATE-DERIVED DISACCHARIDES [J].