Analysis of ABCC6 (MRP6) in normal human tissues

被引：55

作者：

Beck, K ^{[1
]}

Hayashi, K

Dang, K

Hayashi, M

Boyd, CD

机构：

[1] Univ New S Wales, Sch Med Sci, Ctr Vasc Res, Sydney, NSW 2052, Australia

[2] Univ Hawaii, Pacific Biomed Res Ctr, Lab Matrix Pathobiol, Honolulu, HI 96822 USA

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2005年 / 123卷 / 4-5期

关键词：

ABCC6; MRP6; pseudoxanthoma elasticum; tissue distribution; gastrin cells;

D O I：

10.1007/s00418-004-0744-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To determine the tissue distribution of the ABC transporter ABCC6 in normal human tissues, we analyzed tissue arrays for the presence of ABCC6 mRNA by in situ hybridization and ABCC6 protein by immunohistochemistry using the polyclonal antibody HB-6. We detected ABCC6 mRNA and protein in various epithelial cells of exocrine and endocrine tissues, such as acinar cells in the pancreas, mucosal cells of the intestine and follicular epithelial cells of the thyroid. We obtained a very strong immunostaining for enteroendocrine G cells in the stomach. In addition, ABCC6 mRNA and protein were present in most neurons of the brain, in alveolar macrophages in the lungs and lymphocytes in the lymph node. Immunohistochemisty using the monoclonal antibody M6II-31 confirmed the widespread tissue distribution of ABCC6. The physiological substrate(s) of ABCC6 are yet unknown, but we suggest that ABCC6 fulfills multiple functions in different tissues. The strong immunostaining for ABCC6 in G cells suggests that it plays an important role in these endocrine cells.

引用

页码：517 / 528

页数：12

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