Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification, and transport

被引：400

作者：

Mathews, H ^{[1
]}

Clendennen, SK ^{[1
]}

Caldwell, CG ^{[1
]}

Liu, XL ^{[1
]}

Connors, K ^{[1
]}

Matheis, N ^{[1
]}

Schuster, DK ^{[1
]}

Menasco, DJ ^{[1
]}

Wagoner, W ^{[1
]}

Lightner, J ^{[1
]}

Wagner, DR ^{[1
]}

机构：

[1] Exelixis Plant Sci Inc, Portland, OR 97224 USA

来源：

PLANT CELL | 2003年 / 15卷 / 08期

关键词：

D O I：

10.1105/tpc.012963

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activation tagging to identify genes that regulate metabolic pathways. One of the activation-tagged insertion lines (ant1) showed intense purple pigmentation from the very early stage of shoot formation in culture, reflecting activation of the biosynthetic pathway leading to anthocyanin accumulation. The purple coloration resulted from the overexpression of a gene that encodes a MYB transcription factor. Vegetative tissues of ant1 plants displayed intense purple color, and the fruit showed purple spotting on the epidermis and pericarp. The gene-to-trait relationship of ant1 was confirmed by the overexpression of ANTI in transgenic tomato and in tobacco under the control of a constitutive promoter. Suppression subtractive hybridization and RNA hybridization analysis of the purple tomato plants indicated that the overexpression of ANTI caused the upregulation of genes that encode proteins in both the early and later steps of anthocyanidin biosynthesis as well as genes involved in the glycosylation and transport of anthocyanins into the vacuole.

引用

页码：1689 / 1703

页数：15

共 51 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].