Construction of improved vectors for protein production in Pseudomonas aeruginosa

被引：62

作者：

Watson, AA ^{[1
]}

Alm, RA ^{[1
]}

Mattick, JS ^{[1
]}

机构：

[1] UNIV QUEENSLAND, CTR MOLEC & CELLULAR BIOL, BRISBANE, QLD 4072, AUSTRALIA

来源：

GENE | 1996年 / 172卷 / 01期

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

expression vector; protein production; T7; promoter; chloramphenicol acetyl transferase; T7 RNA polymerase;

D O I：

10.1016/0378-1119(96)00026-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the construction of two cloning vectors that are based on the Pseudomonas-Escherichia shuttle vector, pUCP19, The new vectors, pUCPKS and pUCPSK, contain a significantly expanded multiple cloning site (MCS) with an adjacent T7 promoter sequence, In conjunction with specifically engineered host strains encoding an inducible T7 RNA polymerase, these vectors allow the controlled production of plasmid-encoded proteins in both Escherichia coli and Pseudomonas aeruginosa to analyse the spectrum of products encoded by cloned segments of DNA. The usefulness of these vectors was demonstrated by expressing the chloramphenicol acetyltransferase (CAT)-encoding gene.

引用

页码：163 / 164

页数：2

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