Priming for enhanced defence responses by specific inhibition of the Arabidopsis response to coronatine

被引:46
作者
Tsai, Chia-Hong [2 ,3 ]
Singh, Prashant [2 ,3 ]
Chen, Ching-Wei [2 ,3 ]
Thomas, Jerome [4 ]
Weber, Johann [4 ]
Mauch-Mani, Brigitte [1 ]
Zimmerli, Laurent [2 ,3 ]
机构
[1] Univ Neuchatel, Mol & Cellular Biol Lab, CH-2009 Neuchatel, Switzerland
[2] Natl Taiwan Univ, Dept Life Sci, Taipei 106, Taiwan
[3] Natl Taiwan Univ, Inst Plant Biol, Taipei 106, Taiwan
[4] Univ Lausanne, Ctr Integrat Genom, Lausanne Genom Technol Facil, CH-1015 Lausanne, Switzerland
基金
瑞士国家科学基金会;
关键词
Arabidopsis thaliana; beta-aminobutyric acid; priming; coronatine; jasmonic acid; defence response; BETA-AMINOBUTYRIC ACID; SYSTEMIC ACQUIRED-RESISTANCE; PLANT IMMUNE-RESPONSES; PV. TOMATO DC3000; PSEUDOMONAS-SYRINGAE; INNATE IMMUNITY; SIGNALING PATHWAYS; DEFICIENT MUTANTS; MICROARRAY DATA; CROSS-TALK;
D O I
10.1111/j.1365-313X.2010.04436.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>The priming agent beta-aminobutyric acid (BABA) is known to enhance Arabidopsis resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 by potentiating salicylic acid (SA) defence signalling, notably PR1 expression. The molecular mechanisms underlying this phenomenon remain unknown. A genome-wide microarray analysis of BABA priming during Pst DC3000 infection revealed direct and primed up-regulation of genes that are responsive to SA, the SA analogue benzothiadiazole and pathogens. In addition, BABA was found to inhibit the Arabidopsis response to the bacterial effector coronatine (COR). COR is known to promote bacterial virulence by inducing the jasmonic acid (JA) response to antagonize SA signalling activation. BABA specifically repressed the JA response induced by COR without affecting other plant JA responses. This repression was largely SA-independent, suggesting that it is not caused by negative cross-talk between SA and JA signalling cascades. Treatment with relatively high concentrations of purified COR counteracted BABA inhibition. Under these conditions, BABA failed to protect Arabidopsis against Pst DC3000. BABA did not induce priming and resistance in plants inoculated with a COR-deficient strain of Pst DC3000 or in the COR-insensitive mutant coi1-16. In addition, BABA blocked the COR-dependent re-opening of stomata during Pst DC3000 infection. Our data suggest that BABA primes for enhanced resistance to Pst DC3000 by interfering with the bacterial suppression of Arabidopsis SA-dependent defences. This study also suggests the existence of a signalling node that distinguishes COR from other JA responses.
引用
收藏
页码:469 / 479
页数:11
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