Influence of cucumariosides upon intracellular [Ca 2+]i and lysosomal activity of macrophages

被引:23
作者
Agafonova, IG [1 ]
Aminin, DL [1 ]
Avilov, SA [1 ]
Stonik, VA [1 ]
机构
[1] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far Eastern Branch, Vladivostok 690022, Russia
关键词
sea cucumber; triterpene glycosides; cucumariosides; Ca2+ signaling; cytotoxicity; immunomodulation; lysosomal activity; mechanism of action;
D O I
10.1021/jf034439x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Biological effects of the triterpene glycosides, cucumariosides A(2)-2 and A(7)-1 from the edible sea cucumber Cucumaria japonica and their aglycones were investigated using embryos of the sea urchin Strongylocentrotus nudus and the BALB/C line mouse peritoneal macrophages. Cucumariosides were highly cytotoxic in a sea urchin embryo development test with EC50 values of 0.3 and 1.98 mug/mL, respectively. The aglycone was completely lacking in cytotoxicity. In subtoxic concentrations (0.001-0.1 mug/mL), cucumarioside A(2)-2 showed more then 2-fold stimulation of lysosomal activity and induced a rapid short-term increase in cytosolic Ca2+ Content in mouse macrophages. The maximal stimulatory effect was detected after 1-2 h of cultivation of cells with this glycoside. Cucumarioside A(7)-1 demonstrated more weak effects and even slightly inhibited lysosomal activity, while the aglycone was completely ineffective. At the toxic concentration (1 mug/mL), cucumarioside A(2)-2 induced the sharp irreversable increase of intracellular Ca2+ concentration. We suggested that cucumariosides, especially A(2)-2, may act as Ca2+ agonists due to their membranolytic properties.
引用
收藏
页码:6982 / 6986
页数:5
相关论文
共 33 条
[1]  
Allison A., 1969, LYSOSOMES BIOLOGY PA, V2, P600
[2]  
Aminin D. L., 2001, J Med Food, V4, P127, DOI 10.1089/109662001753165701
[3]   The effect of pH on biological activity of plant cytotoxin cauloside C [J].
Aminin, DL ;
Agafonova, IG ;
Gnedoi, SN ;
Strigina, LI ;
Anisimov, MM .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY, 1999, 122 (01) :45-51
[4]  
Anisimov M.M, 1987, BIOL NAUKI, V10, P49
[5]  
Avilov S.A., 1990, KHIM PRIR SOEDIN, V6, P787
[6]   STRUCTURE OF CUCUMARIOSIDE G(2), A NOVEL NONHOLOSTANE GLYCOSIDE FROM THE SEA-CUCUMBER EUPENTACTA-FRAUDATRIX [J].
AVILOV, SA ;
KALININ, VI ;
MAKARIEVA, TN ;
STONIK, VA ;
KALINOVSKY, AI ;
RASHKES, YW ;
MILGROM, YM .
JOURNAL OF NATURAL PRODUCTS, 1994, 57 (08) :1166-1171
[7]  
AWANG DVC, 1999, PERSPECTIVES NEW CRO, P450
[8]   Elementary and global aspects of calcium signalling [J].
Berridge, MJ .
JOURNAL OF PHYSIOLOGY-LONDON, 1997, 499 (02) :291-306
[9]   Cooking with calcium: The recipes for composing global signals from elementary events [J].
Bootman, MD ;
Berridge, MJ ;
Lipp, P .
CELL, 1997, 91 (03) :367-373
[10]  
Drozdova O.A., 1993, KHIM PRIR SOEDIN, V3, P369