Caged Q-rhodamine dextran: A new photoactivated fluorescent tracer

被引：51

作者：

Gee, KR

Weinberg, ES

Kozlowski, DJ

机构：

[1] Mol Probes Inc, Eugene, OR 97402 USA

[2] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 16期

关键词：

D O I：

10.1016/S0960-894X(01)00421-8

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

An amine-reactive caged rhodamine was synthesized and conjugated to aminodextran. The resulting tracer was injected into a single cell zebrafish embryo, and a portion of the tracer was photolyzed in a single cell after development. The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division. (C) 2001 Elsevier Science Ltd. All rights reserved.

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页码：2181 / 2183

页数：3

相关论文

共 11 条

[1] Dynamic deformation visualization in swelling of polymer gels [J].

Achilleos, EC ;

Prud'homme, RK ;

Christodoulou, KN ;

Gee, KR ;

Kevrekidis, IG .

CHEMICAL ENGINEERING SCIENCE, 2000, 55 (17) :3335-3340

[2]

Haugland R.P., 1996, Handbook of Fluorescent Probes and Research Chemicals

[3]

Kozlowski DJ, 2000, METH MOL B, V135, P349

[4] Regional cell movement and tissue patterning in the zebrafish embryo revealed by fate mapping with caged fluorescein [J].

Kozlowski, DJ ;

Murakami, T ;

Ho, RK ;

Weinberg, ES .

BIOCHEMISTRY AND CELL BIOLOGY, 1997, 75 (05) :551-562

[5] PHOTOACTIVABLE FLUOROPHORES .3. SYNTHESIS AND PHOTOACTIVATION OF FLUOROGENIC DIFUNCTIONALIZED FLUORESCEINS [J].

KRAFFT, GA ;

SUTTON, WR ;

CUMMINGS, RT .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (01) :301-303

[6]

LEMPERT WR, 1995, EXP FLUIDS, V18, P249

[7] POLEWARDS MICROTUBULE FLUX IN THE MITOTIC SPINDLE - EVIDENCE FROM PHOTOACTIVATION OF FLUORESCENCE [J].

MITCHISON, TJ .

JOURNAL OF CELL BIOLOGY, 1989, 109 (02) :637-652

[8] The progeny of wingless-expressing cells deliver the signal at a distance in Drosophila embryos [J].

Pfeiffer, S ;

Alexandre, C ;

Calleja, M ;

Vincent, JP .

CURRENT BIOLOGY, 2000, 10 (06) :321-324

[9] ACTIN MICROFILAMENT DYNAMICS IN LOCOMOTING CELLS [J].

THERIOT, JA ;

MITCHISON, TJ .

NATURE, 1991, 352 (6331) :126-131

[10] Purification and assay of the platelet Arp2/3 complex [J].

Welch, MD ;

Mitchison, TJ .

MOLECULAR MOTORS AND THE CYTOSKELETON, PT B, 1998, 298 :52-61