A set of commercially available fluorescent in-situ hybridization probes efficiently detects cytogenetic abnormalities in patients with chronic lymphocytic leukemia

被引：27

作者：

Goorha, S

Glenn, MJ

Drozd-Borysiuk, E

Chen, Z

机构：

[1] Univ Utah, Sch Med, Div Med Genet, Dept Pediat,Dept Internal Med, Salt Lake City, UT USA

[2] Univ Utah, Sch Med, Div Med Genet, Dept Pediat,Div Clin Oncol, Salt Lake City, UT USA

来源：

GENETICS IN MEDICINE | 2004年 / 6卷 / 01期

关键词：

FISH; molecular cytogenetics; B-CLL; CD38; expression; hematological malignancy;

D O I：

10.1097/01.GIM.0000105741.57923.08

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: To investigate a simplified panel of fluorescent in-situ hybridization (FISH) probes for evaluation of patients with chronic lymphocytic leukemia (CLL) and to correlate results from this technique with known prognostic factors. Methods: We retrospectively reviewed the FISH and conventional cytogenetic results, and clinical and laboratory data of 44 patients with CLL. Results: FISH was more sensitive than conventional cytogenetics in detecting genomic aberrations (75% vs. 16%, P < 0.0001). Trisomy 12 was significantly correlated with the cell surface marker of CD38 expression (P = 0.0017). Conclusion: This FISH panel reliably detects prognostically important genomic abnormalities in CLL and is suitable for widespread use.

引用

页码：48 / 53

页数：6

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