Studies of interaction between Safranine T and double helix DNA by spectral methods

被引：178

作者：

Cao, Y ^{[1
]}

He, XW ^{[1
]}

机构：

[1] Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 1998年 / 54卷 / 06期

基金：

中国国家自然科学基金;

关键词：

DNA; intercalative binding; Safranine T; phenazium dye; fluorescence quenching;

D O I：

10.1016/S1386-1425(97)00277-1

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In this paper, the DNA affinity properties of Safranine T (ST), which features a phenazinyl group, were studied. The studies indicated that ST could intercalate into the stack base pairs of DNA. Intrinsic binding constants obtained by different spectral methods were consistent within experimental errors. They were of the order of 10(4) M-1 in DNA base pairs, and the binding site size was about 7 in DNA base pairs. Studies of fluorescence quenching by anionic quenchers and melting temperature of DNA all supported the intercalative binding of ST with DNA. The experiments also showed that electrostatic binding played an important role in the interaction of ST with DNA. This research offers a new intercalation functional group to DNA-targeted drug design. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：883 / 892

页数：10

共 22 条

[1] ENANTIOSELECTIVITY IN THE INTERACTION OF CALF THYMUS DNA WITH ACRIDINE-DERIVATIVES HAVING AN AMINO ESTER OR AMINO ALCOHOL SUBSTITUENT [J].