Real-time broad-range PCR versus blood culture.: A prospective pilot study in pediatric cancer patients with fever and neutropenia

被引：11

作者：

Ammann, Roland A. ^{[1
]}

Zucol, Franziska

Aebi, Christoph

Niggli, Felix K.

Kuehne, Thomas

Nadal, David

机构：

[1] Univ Bern, Dept Pediat, CH-3010 Bern, Switzerland

[2] Univ Zurich, Childrens Hosp, Div Infect Dis, CH-8006 Zurich, Switzerland

[3] Univ Bern, Inst Infect Dis, CH-3010 Bern, Switzerland

[4] Univ Zurich, Div Oncol, Childrens Hosp, CH-8006 Zurich, Switzerland

[5] Univ Basel, Childrens Hosp, CH-4003 Basel, Switzerland

来源：

SUPPORTIVE CARE IN CANCER | 2007年 / 15卷 / 06期

关键词：

bacteremia; broad-range; fever; neutropenia; polymerase chain reaction;

D O I：

10.1007/s00520-007-0235-x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Materials and methods In a pilot study, results of real-time broad-range (16S rRNA) polymerase chain reaction (PCR) performed on 45 blood samples of pediatric cancer patients with fever and neutropenia were compared with blood culture results. Results The PCR assay used, having proven a high sensitivity in artificially spiked blood samples, was positive in only three of ten blood culture-positive samples, and it was positive in 10 of 35 (29%) culture-negative samples. Conclusion This broad-range PCR assay, which may identify not-grown bacteria potentially contributing to fever, needs improvement in sensitivity, and different reasons for positive PCR in negative blood culture samples need to be assessed before clinical application.

引用

收藏

页码：637 / 641

页数：5

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