Centromeric protein B null mice are viable with no apparent abnormalities

被引:105
作者
Perez-Castro, AV [1 ]
Shamanski, FL
Meneses, JJ
Lovato, TL
Vogel, KG
Moyzis, RK
Pedersen, R
机构
[1] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA
[2] Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, Reprod Genet Unit, San Francisco, CA 94143 USA
[3] Univ Calif Irvine, Dept Biol Chem, Irvine, CA 92697 USA
关键词
centromere; CENP-B; gene targeting; mitosis; meiosis;
D O I
10.1006/dbio.1998.9005
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The centromere protein B (CENP-B) is a centromeric DNA/binding protein. It recognizes a 17-bp sequence motif called the CENP-B box, which is found in the centromeric region of most chromosomes. It binds DNA through its amino terminus and dimerizes through its carboxy terminus. CENP-B protein has been proposed to perform a vital role in organizing chromatin structures at centromeres. However, other evidence does not agree with this view. For example, CENP-B is found at inactive centromeres on stable dicentric chromosomes, and also mitotically stable chromosomes lacking a-satellite DNA have been reported. To address the biological function of CENP-B,we generated mouse null mutants of CENP-B by homologous recombination. Mice lacking CENP-B were viable and fertile, indicating that mice without CENP-B undergo normal somatic and germline development. Thus, both mitosis and meiosis are able to proceed normally in the absence of CENP-B. (C) 1998 Academic Press.
引用
收藏
页码:135 / 143
页数:9
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