Direct transformation of site-saturation libraries in Bacillus subtilis

被引：3

作者：

Amin, NS ^{[1
]}

Wong, S ^{[1
]}

Schellenberger, V ^{[1
]}

机构：

[1] Genecor Int, Palo Alto, CA 94304 USA

来源：

BIOTECHNIQUES | 2003年 / 35卷 / 06期

关键词：

D O I：

10.2144/03356bm02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：1134 / +

页数：4

共 10 条

[1] RELATIONSHIP BETWEEN MOLECULAR-STRUCTURE AND TRANSFORMATION EFFICIENCY OF SOME S AUREUS PLASMIDS ISOLATED FROM B-SUBTILIS [J].

CANOSI, U ;

MORELLI, G ;

TRAUTNER, TA .

MOLECULAR & GENERAL GENETICS, 1978, 166 (03) :259-267

[2] MARKER RESCUE TRANSFORMATION BY LINEAR PLASMID DNA IN BACILLUS-SUBTILIS [J].

CONTENTE, S ;

DUBNAU, D .

PLASMID, 1979, 2 (04) :555-571

[3]

DIAZTORRES MR, 2002, WIPO PCT PUBLICATION

[4]

EHRLICH SD, 1978, P NATL ACAD SCI USA, V75, P1433, DOI 10.1073/pnas.75.3.1433

[5] Regulatory inputs for the synthesis of ComK, the competence transcription factor of Bacillus subtilis [J].

Hahn, J ;

Luttinger, A ;

Dubnau, D .

MOLECULAR MICROBIOLOGY, 1996, 21 (04) :763-775

[6] Creating random mutagenesis libraries using megaprimer PCR of whole plasmid [J].

Miyazaki, K ;

Takenouchi, M .

BIOTECHNIQUES, 2002, 33 (05) :1033-+

[7] DIFFERENT SPECIFIC ACTIVITIES OF THE MONOMERIC AND OLIGOMERIC FORMS OF PLASMID DNA IN TRANSFORMATION OF B-SUBTILIS AND ESCHERICHIA-COLI [J].

MOTTES, M ;

GRANDI, G ;

SGARAMELLA, V ;

CANOSI, U ;

MORELLI, G ;

TRAUTNER, TA .

MOLECULAR & GENERAL GENETICS, 1979, 174 (03) :281-286

[8] Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization [J].

Shafikhani, S ;

Siegel, RA ;

Ferrari, E ;

Schellenberger, V .

BIOTECHNIQUES, 1997, 23 (02) :304-310

[9] DIRECTED EVOLUTION OF A SUBTILISIN WITH CALCIUM-INDEPENDENT STABILITY [J].

STRAUSBERG, SL ;

ALEXANDER, PA ;

GALLAGHER, DT ;

GILLILAND, GL ;

BARNETT, BL ;

BRYAN, PN .

BIO-TECHNOLOGY, 1995, 13 (07) :669-673

[10] SITE-DIRECTED MUTAGENESIS OF DOUBLE-STRANDED DNA BY THE POLYMERASE CHAIN-REACTION [J].

WEINER, MP ;

COSTA, GL ;

SCHOETTLIN, W ;

CLINE, J ;

MATHUR, E ;

BAUER, JC .

GENE, 1994, 151 (1-2) :119-123

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