Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes

被引：7

作者：

Cerqueira, GC ^{[1
]}

DaRocha, WD ^{[1
]}

Campos, PC ^{[1
]}

Zouain, CS ^{[1
]}

Teixeira, SMR ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, ICB, Dept Bioquim & Immunol, BR-31270010 Belo Horizonte, MG, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2005年 / 100卷 / 04期

关键词：

Trypanosoma cruzi; gene expression; amastigotes; antigens;

D O I：

10.1590/S0074-02762005000400008

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11% of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9% of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54% of all immunoselected clones). However although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients.

引用

页码：385 / 389

页数：5

共 26 条

[1] The mucin-like glycoprotein super-family of Trypanosoma cruzi:: structure and biological roles [J].

Acosta-Serrano, A ;

Almeida, IC ;

Freitas, LH ;

Yoshida, N ;

Schenkman, S .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 114 (02) :143-150

[2] Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries [J].

Agüero, F ;

Ben Abdellah, K ;

Tekiel, V ;

Sánchez, DO ;

González, A .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2004, 136 (02) :221-225

[3] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[4] Immunization with cDNA expressed by amastigotes of Trypanosoma cruzi elicits protective immune response against experimental infection [J].

Boscardin, SB ;

Kinoshita, SS ;

Fujimura, AE ;

Rodrigues, MM .

INFECTION AND IMMUNITY, 2003, 71 (05) :2744-2757

[5] Identification of transcribed sequences (ESTs) in the Trypanosoma cruzi genome project [J].

Brandao, A ;

Urmenyi, R ;

Rondinelli, E ;

Gonzalez, A ;

deMiranda, AB ;

Degrave, W .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1997, 92 (06) :863-866

[6] BIOLOGY OF TRYPANOSOMA-CRUZI [J].

BRENER, Z .

ANNUAL REVIEW OF MICROBIOLOGY, 1973, 27 :347-382

[7] Characterization of domains of the phosphoriboprotein P0 of Plasmodium falciparum [J].

Chatterjee, S ;

Singh, S ;

Sohoni, R ;

Kattige, V ;

Deshpande, C ;

Chiplunkar, S ;

Kumar, N ;

Sharma, S .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 107 (02) :143-154

[8] ESCHERICHIA-COLI PROTEINS, INCLUDING RIBOSOMAL-PROTEIN S12, FACILITATE IN-VITRO SPLICING OF PHAGE-T4 INTRONS BY ACTING AS RNA CHAPERONES [J].

COETZEE, T ;

HERSCHLAG, D ;

BELFORT, M .

GENES & DEVELOPMENT, 1994, 8 (13) :1575-1588

[9] Characterization of cDNA clones encoding ribonucleoprotein antigens expressed in Trypanosoma cruzi amastigotes [J].

DaRocha, W ;

Bartholomeu, DC ;

Macêdo, CDS ;

Horta, MF ;

Cunha-Neto, E ;

Donelson, JE ;

Teixeira, SMR .

PARASITOLOGY RESEARCH, 2002, 88 (04) :292-300

[10] Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment [J].

Ewing, B ;

Hillier, L ;

Wendl, MC ;

Green, P .

GENOME RESEARCH, 1998, 8 (03) :175-185

← 1 2 3 →