cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription

被引:69
作者
Zhou, YD
Sun, ZM
Means, AR
SassoneCorsi, P
Bernstein, KE
机构
[1] DUKE UNIV,MED CTR,DEPT PHARMACOL,DURHAM,NC 27710
[2] INST GENET & BIOL MOL & CELLULAIRE,F-67404 ILLKIRCH GRAFFENS,FRANCE
关键词
cAMP-response element modulator; spermatogenesis;
D O I
10.1073/pnas.93.22.12262
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Testis angiotensin-converting enzyme (ACE) is a unique form of ACE, only produced by male germ cells, and results from a testis-specific promoter found within the ACE gene. We have investigated the role of cAMP-response element modulator (CREM)tau in testis ACE transcription. In gel shift experiments, testes nuclear proteins retard an oligonucleotide containing the cAMP-response element (CRE) found at position -55 in the testis ACE promoter, anti-CREM antibody supershifts this complex. Competitive gel shift shows that recombinant CREM tau protein and testes nuclear proteins have a similar specificity of binding to the testis ACE CRE. Functional analysis using in vitro transcription and transfection studies also demonstrate that CREM tau protein is a transcriptional activator of the testis ACE promoter. Western blot analysis identifies CREM tau protein in the protein-DNA complex formed between nuclear proteins and the testis ACE CRE motif. This analysis also identified other CREM isoforms in the gel-shifted complex, which are thought to be CREM tau 1/2, CREM alpha/beta, and S-CREM. These data indicate that CREM tau isoforms play an important role as a positive regulator in the tissue-specific expression of testis ACE.
引用
收藏
页码:12262 / 12266
页数:5
相关论文
共 31 条
[1]  
Ausubel FM., 1993, Current Protocols in Molecular Biology
[2]  
BERNSTEIN KE, 1992, SEMIN NEPHROL, V12, P524
[3]   CONCENTRATIONS OF ANGIOTENSIN-CONVERTING ENZYME IN TISSUES OF RAT [J].
CUSHMAN, DW ;
CHEUNG, HS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 250 (01) :261-+
[4]   INDUCTION OF CREM ACTIVATOR PROTEINS IN SPERMATIDS - DOWN-STREAM TARGETS AND IMPLICATIONS FOR HAPLOID GERM-CELL DIFFERENTIATION [J].
DELMAS, V ;
VANDERHOORN, F ;
MELLSTROM, B ;
JEGOU, B ;
SASSONECORSI, P .
MOLECULAR ENDOCRINOLOGY, 1993, 7 (11) :1502-1514
[5]   ALTERNATIVE USAGE OF INITIATION CODONS IN MESSENGER-RNA ENCODING THE CAMP-RESPONSIVE-ELEMENT MODULATOR GENERATES REGULATORS WITH OPPOSITE FUNCTIONS [J].
DELMAS, V ;
LAOIDE, BM ;
MASQUILIER, D ;
DEGROOT, RP ;
FOULKES, NS ;
SASSONECORSI, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4226-4230
[6]   MOLECULAR-CLONING OF HUMAN TESTICULAR ANGIOTENSIN-CONVERTING ENZYME - THE TESTIS ISOZYME IS IDENTICAL TO THE C-TERMINAL HALF OF ENDOTHELIAL ANGIOTENSIN-CONVERTING ENZYME (POLYMERASE CHAIN-REACTION ALTERNATIVE SPLICING) [J].
EHLERS, MRW ;
FOX, EA ;
STRYDOM, DJ ;
RIORDAN, JF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) :7741-7745
[7]   DEVELOPMENTAL SWITCH OF CREM - FUNCTION DURING SPERMATOGENESIS - FROM ANTAGONIST TO ACTIVATOR [J].
FOULKES, NS ;
MELLSTROM, B ;
BENUSIGLIO, E ;
SASSONECORSI, P .
NATURE, 1992, 355 (6355) :80-84
[8]   CREM GENE - USE OF ALTERNATIVE DNA-BINDING DOMAINS GENERATES MULTIPLE ANTAGONISTS OF CAMP-INDUCED TRANSCRIPTION [J].
FOULKES, NS ;
BORRELLI, E ;
SASSONECORSI, P .
CELL, 1991, 64 (04) :739-749
[9]   PITUITARY-HORMONE FSH DIRECTS THE CREM FUNCTIONAL SWITCH DURING SPERMATOGENESIS [J].
FOULKES, NS ;
SCHLOTTER, F ;
PEVET, P ;
SASSONECORSI, P .
NATURE, 1993, 362 (6417) :264-267
[10]   THE CYCLIC-AMP RESPONSE ELEMENTS OF THE GENES FOR ANGIOTENSIN-CONVERTING ENZYME AND PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP) CAN MEDIATE TRANSCRIPTIONAL ACTIVATION BY CREM-TAU AND CREM-ALPHA [J].
GORAYA, TY ;
KESSLER, SP ;
STANTON, P ;
HANSON, RW ;
SEN, GC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (32) :19078-19085